Robert Ingham, PhD
Dept. of Medical Microbiology & Immunology
University of Alberta
Faculty of Medicine & Dentistry
6-142H Katz Group Centre
T6G 2E1 Edmonton, AB
Ph : (780) 248-1980
Fx : (780) 492-7521
- Associate Professor, Dept. of Medical Microbiology & Immunology
- Immunology and Infection (IMIN) Program Advisor
Signal transduction pathways control aspects of lymphocyte biology including development, activation, survival, and migration. The importance of these pathways is underscored by the fact that when these pathways are dysregulated, immunodeficiency, autoimmunity, or cancer can result. My laboratory studies the molecular mechanisms of lymphocyte signalling, and we are particularly interested in the role protein-protein interactions and post-translational modifications play in regulating these events. Specific interests of the laboratory include: elucidating how the Epstein-Barr virus protein, LMP2A, co-opts lymphocyte signalling pathways, the function of protein ubiquitylation in lymphocyte signalling, and understanding how dysregulated signalling contributes to the pathogenesis of B and T cell lymphomas. We use a variety of molecular biology and biochemical techniques as well as cell-based assays in our studies..
- Bettina Bareiss (Graduate Student)
- Zuoqiao Wu (Graduate Student)
- JingXi Zhang (Graduate Student)
Publications (trainees underlined)
Lee JK*, Pearson JD*, Maser BE, Ingham RJ. (2013). Cleavage of the JunB Transcription Factor by Caspases Generates a Carboxy-Terminal Fragment that Inhibits Activator Protein-1 Transcriptional Activity. Journal of Biological Chemistry. Jun 9. [Epub ahead of print]
Adler JJ, Heller BL, Bringman LR, Ranahan WP, Cocklin RR, Goebl MG, Oh M, Lim HS, Ingham RJ, Wells CD. Amot130 Adapts Atrophin-1 Interacting Protein 4 to Inhibit Yes-associated Protein Signaling and Cell Growth. Journal of Biological Chemistry. May 24;288(21):15181-93.
Lai, R and Ingham RJ. (2012). The pathobiology of the oncogenic tyrosine kinase NPM-ALK – a brief update. Therapeutic Advances in Hematology. Apr;4(2):119-31. (Review Article)
Hegazy SA, Alshareef A, Gelebart P, Anand M, Armanious H, Ingham RJ, and Lai R. Disheveled proteins promote cell growth and tumorigenicity in ALK-positive anaplastic large cell lymphoma. Cellular Signalling. 25(1):295-307.
Gelebart P, Hegazy SA, Wang P, Bone KM, Anand M, Sharon D, Hitt M, Pearson JD, Ingham RJ, Ma Y, Lai R (2012). Aberrant expression and biological significance of Sox2, an embryonic stem cell transcriptional factor, in ALK-positive anaplastic large cell lymphoma. Blood Cancer Journal. Aug 10;2:e82.
Wu F, Zhang J, Wang P, Ye X, Jung K, Bone KM, Pearson JD, Ingham RJ, McMullen TP, Ma Y, Lai R. (2012). Identification of two novel phenotypically distinct breast cancer cell subsets based on Sox2 transcription activity. Cellular Signalling. 24(11):1989-98.
Zhang J, Wang P, Dykstra M, Gelebart P, Williams D, Ingham R, Ekpe Adewuyi E, Lai R, McMullen T. (2012). Platelet Derived Growth Factor Receptor-α Promotes Lymphatic Metastases in Papillary Thyroid Cancer. Journal of Pathology. 228(2):241-50.
Pearson JD, Mohammed Z, Bacani JTC, Lai R, Ingham RJ. (2012). The heat shock protein-90 co-chaperone, Cyclophilin 40, promotes ALK-positive, anaplastic large cell lymphoma viability and its expression is regulated by the NPM-ALK oncoprotein. BMC Cancer. 12:229.
Pearson JD, Lee JKH, Bacani JTC, Lai R, Ingham RJ. (2012). NPM-ALK: the prototypic member of a family of oncogenic tyrosine kinases. Journal of Signal Transduction. 22012:123253. (Review Article)
Zhang J, Wang P, Wu F, Li M, Sharon D, Ingham RJ, Hitt M, McMullen TP, Lai R. (2012) Aberrant expression of the transcriptional factor Twist1 promotes invasiveness in ALK-positive anaplastic large cell lymphoma. Cellular Signalling. 24(4):852-8
Pearson JD, Lee JK, Bacani JT, Lai R, Ingham RJ. (2011) NPM-ALK and the JunB transcription factor regulate the expression of cytotoxic molecules in ALK-positive, anaplastic large cell lymphoma. International Journal of Clinical and Experimental Pathology.4(2):124-33.
Wang P, Wu F, Zhang J, McMullen T, Young LC, Ingham RJ, Li L, Lai R. (2011). Serine phosphorylation of NPM-ALK, which is dependent on the auto-activation of the kinase activation loop, contributes to its oncogenic potential. Carcinogenesis. 32(2):146-53.
Hegazy SA, Wang P, Anand M, Ingham RJ, Gelebart P, Lai R. (2010). The tyrosine 343 residue of nucleophosmin (NPM)-anaplastic lymphoma kinase (ALK) is important for its interaction with SHP1, a cytoplasmic tyrosine phosphatase with tumor suppressor functions. Journal of Biological Chemistry. 285(26):19813-20.
Alford SC, Pearson JD, Carette A, Ingham RJ, Howard PL. (2009). Alpha-sarcin catalytic activity is not required for cytotoxicity. BMC Biochemistry. 10:9.